| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rogerson, Annika R (12)
|
VAUN-V |
32.50 |
32.55 |
0.05 |
| 2 |
Czirjak, Sara E (12)
|
VAGATR |
32.61 |
32.66 |
0.05 |
| 3 |
Ismailova, Diana (11)
|
VAGATR |
34.58 |
34.23 |
-0.35 |
| 4 |
Dugas, Georgia K (12)
|
VAACAC |
43.40 |
37.36 |
-6.04 |
| 5 |
McCardle, Reese B (11)
|
VAACAC |
41.25 |
38.66 |
-2.59 |
| 6 |
Brushwood, Madison P (11)
|
VABASS |
38.64 |
39.39 |
0.75 |
| 7 |
Hoang, Angelina (11)
|
VAACAC |
|
39.40 |
- |
| 8 |
Wells, Abigail J (11)
|
VATSU |
|
40.20 |
- |
| 9 |
Segura, Mara J (11)
|
VATSU |
47.10 |
40.73 |
-6.37 |
| 10 |
Barclay, Sydney M (12)
|
VAACAC |
42.75 |
40.84 |
-1.91 |
| 11 |
Mejeur, Hannah J (12)
|
VATSU |
47.79 |
42.75 |
-5.04 |
| 12 |
Turner, Paige Y (11)
|
VATSU |
42.75 |
43.13 |
0.38 |
| 13 |
Semmelhack, Sylvie W (11)
|
VAGATR |
|
43.20 |
- |
| 14 |
McCORKLE, Madeline G (11)
|
VATSU |
|
43.85 |
- |
| 15 |
Hernandez, Maya G (11)
|
VABASS |
53.79 |
44.32 |
-9.47 |
| 16 |
Brooks, Madelyne G (11)
|
VABASS |
48.78 |
44.85 |
-3.93 |
| 17 |
Brice, Madison R (11)
|
VABASS |
|
48.87 |
- |
| 18 |
Martin, Grace E (11)
|
VABASS |
|
58.53 |
- |